I did mine without using threads on the acrylic at all.
My system uses large tubing, but it would work the same with your itty bitty tubes.
I used a 3/4" female to 1" female pipe coupling, and a 3/4" female to 1" male adapter bushing. Drill a hole in the acrylic and slip the smaller bushing through the hole from the inside of the tank (with Goop added) and cement it into the female coupling.
Wait three hours for curing, and it's done. Solid, water-tight, and not half bad looking. Been going strong for two years now.
Another idea is to use any pvc that's roughly the same diameter as the acrylic (so radius' match close).
Cut out a square of the pvc (or a round shape if you prefer) and Goop it in place over a slightly larger than needed hole already made in the acrylic. When the Goop is cured, you can drill and tap the pvc with the threads, not even stressing the acrylic at all.
You can drill and tap the pvc piece beforehand as well. Larger PVC fittings are around 1/4" thick plastic and have plenty of meat for threads. It's also easy to cut threads in, and will not split like the clear stuff can/will.
Taps for 1/4" NPT cost around $8.
Goop btw, is a brand of clear adhesive that's waterproof and sticks pretty darned well to plastics. Either marine or plumber's will do fine, and are identical as near as I can tell except for package color.
Rough up the surfaces before bonding them, and it will hold nicely.
@ Lowe's/Depot.
While I do use a vortex in my res, I don't really recommend having bubbles reaching your intake in the res...that's a sure fire way to kill a pump. Practice it before installing it permanently.