Since I used boiling water to rinse all of my parts after a hot soapy wash out (Dawn w/bleach), I ran for a year without growth in my system using only distilled water. Of course, I also rinse like mad to remove all the soap before the boiling distilled final rinse, and the dawn dishsoap uses a bleach substitute (for those concerned it was that).
Prep and cleaning beforehand is key here, and really needs to be paid attention to. If someone sneezed on a component or machine making your part, or a spore happened to drift into the production area at the right time, you'll most likely have growth(s) sooner or later if you don't clean the daylights out of your parts.
Now for the downside. I have an all copper/ brass/ plastic loop. I had no growth inside my system for that year, but the water started to get a greenish tinge to it and became sort of cloudy looking.
What I did get was corrosion in my main radiator and inside of my MCW6000 waterblock. Nice (albeit tiny) light green chunks of corrosion were flushed from my system when I cleaned it out for a re-fill. The waterblock looked as if it were painted light green inside. Definately copper corrosion, and not an algae attack.
Not much, not like a hand-full of chunks to flush out(big truck radiator here), but definately something I had to remedy.
I now use only a 2-3% solution (or less) of anti-freeze in my loop, just enough to get the beginnings of a color change of the coolant.
I added a splash of Iodine this time also because I wasn't able to disassemble the loop entirely to do my usual near-sterilization.
I've
always used steam distilled water...I accept no substitutes.
Plus my temps have remained the same, then as now.
I've concluded that since water is 1/3 oxygen (just based on numbers, not weight or some such siliness), it's crazy not to expect
and plan for copper oxidation. That's what the AF gets you. You don't need much, a little dab will do ya.