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Defrag is needed for mechanical platter drives because the speed at which the data can be read from the center of the disc vs the outer edge differs. However, I don't think it will be necessary to do that before you clone the HDD because it won't make any impact on the performance or the cloning speed whatsoever.
As for the estimated time, it's hard to say how much exactly it will be, but the calculation provided by @Suppressor1137 are pretty good and will give you a basic idea of how long it will take.
Hope this helps and feel free to ask any questions you may have!
I did not realize an image omitted blank space... Perhaps only unformatted space? Either way this thread has been informative for me as well. ThanksThe drive can also be imaged, instead of cloning. That will only copy the data, and not the whole partition. There are plenty of options out there, at no cost.