I think your problem is that you're not sure if the RAM is damaged or not, since you took out your first motherboard when you plugged it in backwards. If there's visible damage (scorching, scratched or chipped contacts, etc.) to the pins or the chips on the RAM, I wouldn't do it (unless you're eager for a motherboard RMA). It's likely shot.
If it looks OK, I doubt you've managed to fricasee the internals that badly (how did you get it in backwards anyway? They only fit one way...) Test at your own risk.
A computer repair place might have a RAM tester, which would tell you if the stick is still usable without endangering any of your equipment. You could also try the manufacturer if it's still under warranty.