1/4" will work just fine. I have used coldplates as thin as .090" and got good results but temp stability suffers somewhat with a plate that thin. A 10mm (3/8") gives the best tradeoff of temp stability and effeciency. Its all personal preference really.. I prefer temp stability over getting the absolute lowest temp possible so I use 7/16" coldplates for all peltiers 48mm x 48mm and larger.
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